Using 50,000 bone cells and tendon cells per gel, and 100,000 muscle cells per gel, the cells stop multiplying and start forming tissue within two weeks.
Scientific Claim
Cell seeding densities of 50,000 cells per gel for MG-63 and human dermal fibroblasts, and 100,000 cells per gel for Sket.4U cells, achieve homeostasis in a hydrogel model within 14 days.
Original Statement
“For MG-63 and HDF cells, 50,000 cells/gel was chosen as the most suitable cell seeding density. [...] 100,000 cells/gel was chosen as the cell seeding density [for Sket.4U] due to the lack of fibre formation in 2D culture.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study directly measured cell homeostasis at specific densities, so the statement is a factual observation.
Evidence from Studies
Supporting (1)
Triphasic 3D In Vitro Model of Bone-Tendon-Muscle Interfaces to Study Their Regeneration