When grown together in a gel model, bone cells (MG-63) make osteonectin, tendon cells (HDF) make tenomodulin, and muscle cells (Sket.4U) make α-SMA, and these markers are still present after two weeks.
Scientific Claim
MG-63 osteoblast-like cells, human dermal fibroblasts, and Sket.4U skeletal muscle cells co-cultured in a hydrogel model express osteonectin, tenomodulin, and α-SMA markers, respectively, for up to 14 days.
Original Statement
“On day 1, MG-63 cells expressed osteonectin, HDF cells expressed tenomodulin, and Sket.4U cells expressed αSMA. By day 14, osteonectin, tenomodulin, and αSMA were still expressed.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study directly observed marker expression via immunocytochemistry, so stating the presence of markers is a factual observation.
Evidence from Studies
Supporting (1)
Triphasic 3D In Vitro Model of Bone-Tendon-Muscle Interfaces to Study Their Regeneration