When scientists changed one amino acid in the creatine transporter (from alanine to proline at position 285), it made the transporter more sensitive to guanidinoethane sulfonic acid, which blocked the mutant transporter about twice as well as the normal transporter in frog cells.
Scientific Claim
Guanidinoethane sulfonic acid inhibits the A285P mutant of the human CRT-1 creatine transporter in Xenopus oocytes approximately two times more effectively than the wild type, indicating that this mutation affects sensitivity to this specific inhibitor in this experimental system.
Source Excerpt
“In contrast, guanidinoethane sulfonic acid, a potent inhibitor of taurine transport, inhibited the uptake activity of the A285P mutant approx. two times more effectively than that of the wild type.”
Evidence from Studies
Supporting Studies
Molecular characterization of the human CRT-1 creatine transporter expressed in Xenopus oocytes.
The study directly compared the inhibition of the wild-type and A285P mutant creatine transporter by guanidinoethane sulfonic acid in Xenopus oocytes. The finding indicates that the mutation at position 285 specifically affects sensitivity to this inhibitor in this experimental system.