When rats do certain muscle exercises, they get sore deep in their muscles—not on the skin—starting a day later, peaking after 2–3 days, and going away by day 4. This soreness is due to increased...

Even if a rat's muscles aren't actually torn or damaged, it can still feel sore after exercise — this soreness seems to come from nerve sensitivity, not from physical damage, especially when the exercise isn't too intense.

In rats, sore muscles after unusual exercise seem to start with a specific pain signal (called bradykinin) turning on right when the exercise happens. If you block that signal before exercise, the rats don’t get sore — but only if you block it early.

In rats with muscle soreness after exercise, two brain-chemical pathways team up to make muscles extra sensitive to pain, especially when certain pain-related proteins are present at low levels — and they both work through specific pain sensors in the nerves.