In healthy young men, ingesting 45 grams of protein before sleep increases mitochondrial and myofibrillar protein synthesis rates during overnight recovery following a single bout of endurance exercise, compared to placebo, with no significant difference observed between whey and casein protein sources.

Ingesting 45 grams of protein before sleep increases both mitochondrial and myofibrillar protein synthesis rates during overnight recovery in healthy young men after a single bout of endurance exercise, compared to placebo. This effect occurs regardless of whether the protein source is whey or casein, with no significant difference between the two. The increase in mitochondrial protein synthesis (0.087 ± 0.020%·h⁻¹ vs 0.067 ± 0.016%·h⁻¹) and myofibrillar protein synthesis (0.060 ± 0.014%·h⁻¹ vs 0.047 ± 0.011%·h⁻¹) demonstrates that pre-sleep protein effectively supports muscle repair and remodeling processes during sleep.