The Claim

In murine C2C12 skeletal myotubes, treatment with 1,25(OH)2-vitamin D3 at a concentration of 10 nM in combination with leucine and insulin increases the protein fractional synthesis rate by 14–16%, demonstrating a synergistic effect on muscle protein production in this in vitro cell model.

Source: 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C2C12 skeletal myotubes.

What the research says

Supports is higher

Support is ahead, but a single strong opposing study can change this.

Supports
9score
Challenges
0score

These are independent scores, not a percentage. Higher-grade studies count more, so a single strong opposing study can outweigh several weaker ones.

Cause and effect
1 study reviewed
In plain English

When scientists added a form of vitamin D along with leucine and insulin to muscle cells from mice, the cells made more protein than usual—about 14 to 16% more—suggesting these three things work better together than alone.

See the scientific wording

In murine C2C12 skeletal myotubes, 1,25(OH)2-vitamin D3 at 10 nM enhances the protein fractional synthesis rate by 14–16% when combined with leucine and insulin, indicating a synergistic effect on muscle protein production in this cell model.

What the research says

1 study
  1. Study: 1,25(OH)2-vitamin D3 enhances the stimulating effect of leucine and insulin on protein synthesis rate through Akt/PKB and mTOR mediated pathways in murine C2C12 skeletal myotubes.

    The study found that when mouse muscle cells are given vitamin D along with leucine and insulin, they make more protein — exactly as the claim says. The numbers and conditions match perfectly.

Score breakdown, mechanism chain, raw evidence, ideal studies needed & 1 supporting studies

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