The Claim

The E. coli B834 (DE3) assay identifies vitamin B12-producing bacterial strains from the wild microbiome of Caenorhabditis elegans, including four strains previously predicted as producers that are confirmed by experimental growth response.

Source: A simple, fast and inexpensive approach using E. coli to detect and estimate vitamin B12 content in microbial extracts

What the research says

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Description
1 study reviewed
In plain English

A laboratory test using E. coli B834 (DE3) detects bacteria from the gut of Caenorhabditis elegans that produce vitamin B12, and four such bacteria previously predicted to produce it have been confirmed by their ability to support growth in the test system.

See the scientific wording

The E. coli B834 (DE3) assay enables the identification of vitamin B12-producing bacterial strains from the wild microbiome of Caenorhabditis elegans, including four previously predicted producers confirmed by experimental growth response.

Why this might work

A special strain of E. coli cannot make its own methionine unless it gets vitamin B12. When this bacterium is exposed to a sample from the worm's gut, it only grows if the sample contains vitamin B12 made by another bacterium. The amount of growth shows how much vitamin B12 is present, letting scientists find which bacteria in the worm's microbiome are producing it.

Verified mechanismbased on 1 study

What the research says

1 study
  1. Study: A simple, fast and inexpensive approach using E. coli to detect and estimate vitamin B12 content in microbial extracts

    Scientists used a special type of E. coli bacteria that needs vitamin B12 to grow, and they fed it samples from worms in the wild. If the bacteria grew, it meant the sample had B12 — proving which bacteria were making it, just like their genes said they could.

Score breakdown, mechanism chain, raw evidence, ideal studies needed & 1 supporting studies

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