The Study
Probing binding and occlusion of substrate in the human creatine transporter‐1 by computation and mutagenesis
This study is like building a Lego model of a car using pictures and guessing how it works — it doesn’t prove the real car drives, but it might help us guess how the engine could work.
Analysis score
Maximum 0 for a computational/algorithm study.
Where the score came from
Your muscles need creatine to store energy, and a special door (CRT1) lets it in. This study figured out how that door works using computer simulations.
Where does this study sit?
Systematic Reviews & Meta-analyses
Max 100Randomized Trials
Max 90Cohort Studies
Max 72Case-Control
Max 58Cross-Sectional
Max 44Case Reports & Series
Max 30Expert Opinion
Max 50 / 100
Quality score
Based on clinical experience or non-systematic literature reviews. The lowest level of evidence as they are most susceptible to bias and personal perspective.
Key takeaways
Summary
Based on the study abstract and findings.
- 1Yes — understanding how this door breaks helps fix rare genetic diseases that prevent creatine from entering brain and muscle cells.
- 2D458 must be neutral (protonated) to keep the door stable; C144 is not charged as once thought; Y148 holds creatine while sodium pulls the door shut.
Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data
Publication
Journal
Protein Science : A Publication of the Protein Society
Year
2023
Authors
Amy Clarke, Clemens V. Farr, Ali El‐Kasaby, D. Szöllősi, M. Freissmuth, S. Sucic, T. Stockner
Not medical advice. For informational purposes only. Always consult a qualified healthcare professional before making health decisions.