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The Study

Low energy availability increases immune cell formation of reactive oxygen species and impairs exercise performance in female endurance athletes

In simple terms

This study made athletes eat less food for two weeks and saw what happened to their bodies during exercise. Because they switched back and forth between eating enough and not enough, we can say that eating too little probably caused the changes they saw — like feeling more tired and their immune cells making more stress chemicals.

71%

Analysis score

71/ 90

Maximum 90 for a randomized controlled trial.

Where the score came from

Reporting40
Methodology69
Publication100
Statistical77
Study type (basis of the score)
Randomized Controlled Trial
Level 1b - Individual RCT
What’s the bottom line?

When female athletes don't eat enough to match their training, their immune cells start making too much harmful stress gas (H2O2), their stress hormone (cortisol) goes up, and their body stops sending immune soldiers to fight during exercise.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Randomized Trials
Level 1b
71

71 / 100

Quality score

Participants are randomly assigned to treatment or control groups, minimizing bias. The gold standard for testing whether an intervention causes an effect.

Can establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1Yes—this means even after eating properly again, athletes still can't perform at their best, suggesting short-term dieting can leave lasting damage to athletic ability.
  2. 2After 14 days of eating too little: immune cells made 94% more stress enzyme, exercise made them produce 341% more H2O2, cortisol rose 22%, immune cell movement during exercise dropped 25%, and running performance fell 7.8%.
  3. 3After 3 days of eating normally: cortisol and immune markers returned to normal, but performance stayed 6.7% worse.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Redox Biology

Year

2024

Authors

J. S. Jeppesen, Hannah G. Caldwell, Lone O. Lossius, Anna K Melin, L. Gliemann, Jens Bangsbo, Y. Hellsten

Open Access
26 citations
Analysis v5

Related Content

Claims (6)

Assertion

Athletes who do not consume enough energy to meet their training demands have a higher rate of injury compared to those who do.

Correlational
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Assertion

When female endurance athletes consume too few calories for 14 days, their bodies release fewer white blood cells during exercise compared to normal conditions, even though baseline levels remain unchanged.

Mechanistic
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Assertion

After 14 days of low energy intake, female endurance athletes who consume 52 kcal per kilogram of fat-free mass per day for three days return to normal levels of cortisol and inflammatory proteins in their blood, but their exercise performance does not improve.

Causal
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Assertion

In female endurance athletes, consuming too few calories for 14 days increases the production of NADPH oxidase 2 protein and exercise-induced hydrogen peroxide in immune cells, without changing baseline hydrogen peroxide levels or antioxidant enzyme levels.

Mechanistic
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Assertion

In female endurance athletes, consuming only 22 kcal per kilogram of fat-free mass per day for 14 days raises resting cortisol levels by 22% and changes the levels of seven plasma proteins involved in immune regulation—five decrease and two increase—without affecting standard inflammatory markers like hsCRP or IL-6.

Mechanistic
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Assertion

Female endurance athletes who consume too few calories for 14 days perform 7.8% worse in a 20-minute time trial, and this performance drop does not reverse after three days of eating normally, even though cortisol and inflammatory markers return to normal levels.

Causal
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