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The Study

One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event

In simple terms

This study watched how skin proteins changed over a year when people used special creams. It found that certain molecules in the skin moved up or down after using the creams, but it didn’t prove the creams caused those changes—maybe other things like diet or sun exposure played a role too.

36%

Analysis score

36/ 36

Maximum 36 for a cohort study.

Where the score came from

Reporting40
Methodology35
Publication100
Statistical54
Study type (basis of the score)
Cohort Study
Level 2b - Individual cohort study
What’s the bottom line?

These skincare ingredients don’t just smooth wrinkles — they turn on your skin’s energy and protein factories first, then fix its protective barrier over time.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Cohort Studies
Level 2b
36

36 / 100

Quality score

Groups of people are followed over time to see who develops an outcome. Strong for identifying risk factors and associations, but cannot prove causation as firmly as RCTs.

Cannot establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1Yes — these changes explain why skin looks smoother, brighter, and more resilient after months of use, even if wrinkles don’t vanish immediately.
  2. 2At 3 months: 6 glycolytic enzymes and 16 translation factors increased; 14 glycan-degrading enzymes decreased.
  3. 3At 12 months: filaggrin, involucrin, and calmodulin-like protein 3 stayed high; epidermis thickened.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Scientific Reports

Year

2023

Authors

K. Abed, A. Foucher, D. Bernard, E. Tancrède‐Bohin, N. Cavusoglu

Open Access
5 citations
Analysis v5

Related Content

Claims (6)

Assertion

Using tretinoin cream on the skin for one month reduces wrinkles, evens out skin pigmentation, and fades age spots.

Causal
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Assertion

Daily use of 0.3% retinol or 0.025% all-trans-retinoic acid on the skin for one year is associated with increased levels of proteins involved in energy production, protein synthesis, and skin barrier formation, and decreased levels of proteins that break down sugar molecules in the outer skin layer.

Correlational
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Assertion

Topical retinol and retinoic acid reduce the activity of enzymes that break down glycans in the outer layer of skin after three months of use, with retinol having a stronger effect than retinoic acid.

Correlational
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Assertion

When applied to the skin, retinol and retinoic acid trigger changes in proteins and cellular molecules within three months, but visible structural changes like thickening of the skin layer do not become significant until twelve months.

Mechanistic
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Assertion

Applying retinol and retinoic acid to the skin increases the activity of certain metabolic and protein-building molecules in the outer skin layer, reaching highest levels at 3 months and decreasing by 12 months.

Mechanistic
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Assertion

Topical retinol and retinoic acid change the expression levels of specific skin barrier proteins and enzymes in a sustained manner, resulting in structural alterations to the outermost layer of the skin.

Mechanistic
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