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The Study

Zinc isotopes in Late Pleistocene fossil teeth from a Southeast Asian cave setting preserve paleodietary information

In simple terms

This study looked at the chemical fingerprints in ancient animal teeth and found that meat-eaters, plant-eaters, and mixed-eaters had different patterns — like different colors in a paint palette. But it didn’t change what the animals ate to test if the pattern was caused by diet — it just noticed the pattern after the fact.

15%

Analysis score

15/ 44

Maximum 44 for a cross-sectional study.

Where the score came from

Reporting35
Methodology8
Publication100
Statistical77
Study type (basis of the score)
Cross-Sectional Study
Level 4 - Case series
What’s the bottom line?

Scientists studied ancient animal teeth from a cave in Laos to see what they ate by measuring zinc in their enamel, since the old protein in bones was gone.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Cross-Sectional & Case Series
Level 4
15

15 / 100

Quality score

Snapshots of a population at a single point in time, or descriptions of small groups. Can identify correlations and prevalence, but cannot determine cause and effect.

Cannot establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1This means we can now tell if ancient humans or animals ate mostly meat or plants even when bones are too old for protein tests.
  2. 2Carnivores had the lowest zinc levels (0.09‰), herbivores the highest (0.68‰), and omnivores were in between (0.41‰).
  3. 3Omnivores showed the most variation, meaning they ate both plants and meat.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Proceedings of the National Academy of Sciences of the United States of America

Year

2020

Authors

N. Bourgon, K. Jaouen, A. Bacon, K. Jochum, E. Dufour, P. Duringer, J. Ponche, R. Joannes‐Boyau, Quentin Boesch, P. Antoine, Manon Hullot, U. Weis, E. Schulz-Kornas, Manuel Trost, D. Fiorillo, F. Demeter, Elise Patole-Edoumba, L. Shackelford, T. Dunn, A. Zachwieja, Somoh Duangthongchit, Thongsa Sayavonkhamdy, P. Sichanthongtip, Daovee Sihanam, V. Souksavatdy, J. Hublin, T. Tütken

Open Access
57 citations
Analysis v5

Related Content

Claims (7)

Assertion

Throughout most of human evolutionary history, the diet of early human ancestors consisted mainly of animal-based foods, with meat being the main source of nutrition.

Descriptive
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Assertion

Between 1.2 million and a few hundred thousand years ago, early human ancestors like Homo and Neanderthals relied mainly on meat and other animal foods for nutrition, while earlier ancestors like Australopithecus ate mostly plants.

Descriptive
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Assertion

Analysis of zinc isotopes in ancient tooth enamel shows no link to carbon or oxygen isotopes that reflect diet type or climate conditions, meaning zinc isotopes can reliably indicate an organism's position in the food chain without being influenced by those other factors.

Correlational
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Assertion

The zinc isotope signatures in ancient human teeth from Laos have remained unchanged since burial, because their chemical patterns match those of modern teeth and show no link to elements that typically enter bones and teeth after death, even though other organic material in the samples has degraded.

Descriptive
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Assertion

Analysis of zinc isotopes in fossilized teeth from ancient mammals in Laos reveals a predictable pattern: meat-eating animals have lower zinc isotope values than plant-eating animals, with herbivores showing the highest values. This pattern persists even after tens of thousands of years, indicating that zinc isotopes in tooth enamel can record dietary habits when other biological markers like collagen have disappeared.

Descriptive
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Assertion

Analysis of zinc isotopes in fossil teeth from Tam Hay Marklot cave shows that animals with mixed diets had the widest variation in zinc isotope levels, allowing scientists to identify which ancient species ate both plants and animals.

Mechanistic
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