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The Study

ACE gene polymorphisms (rs4340) II and DI are more responsive to the ergogenic effect of caffeine than DD on aerobic power, heart rate, and perceived exertion in a homogeneous Brazilian group of adolescent athletes

In simple terms

This study gave teens caffeine or a fake pill and saw how well they ran, then checked if their genes made a difference. It shows that for these specific teens, caffeine helped some more than others—but it doesn’t prove caffeine works this way for everyone else.

58%

Analysis score

58/ 90

Maximum 90 for a randomized controlled trial.

Where the score came from

Reporting0
Methodology70
Publication100
Statistical54
Study type (basis of the score)
Randomized Controlled Trial
Level 1b - Individual RCT
What’s the bottom line?

Some teens have a gene version that makes caffeine help them run farther and breathe better during sports, while others don't get much benefit.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Randomized Trials
Level 1b
58

58 / 100

Quality score

Participants are randomly assigned to treatment or control groups, minimizing bias. The gold standard for testing whether an intervention causes an effect.

Can establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1Yes — for teens with the right gene, caffeine can make a big difference in endurance, like running nearly 300 meters farther in a high-intensity test.
  2. 2Teens with the II or DI gene version ran 160–286 meters farther and had 1.1–1.4 mL·kg⁻¹·min⁻¹ higher oxygen use after caffeine.
  3. 3Teens with DD gene ran only 31 meters farther.
  4. 484% of II teens improved with caffeine; only 33% of DD teens did.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Brazilian Journal of Medical and Biological Research

Year

2024

Authors

H. Spineli, M. Santos, D. Almeida, D. Gitai, M. Silva-Cavalcante, P. Balikian, T. Ataide-Silva, A. Marinho, F. Sousa, G. Araujo

Open Access
3 citations
Analysis v5

Related Content

Claims (6)

Assertion

In adolescent athletes with the ACE II genotype, consistent caffeine consumption is linked to higher endurance performance and aerobic power only when caffeine is consumed shortly before exercise, and this effect depends on their genetic profile.

Correlational
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Assertion

In adolescent male athletes of Brazilian descent, consuming 6 mg of caffeine per kilogram of body weight increases endurance performance and VO2max only in those with the ACE gene I allele (DI or II genotypes), not in those with the DD genotype.

Causal
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Assertion

Among adolescent male athletes, those with the ACE DD genotype show a lower rate of endurance performance improvement after consuming caffeine compared to those with the DI or II genotype.

Correlational
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Assertion

In adolescent athletes, caffeine changes how hard exercise feels during high-intensity endurance activity, but the effect depends on their ACE gene variant: it makes effort feel harder for those with one variant and easier for those with another.

Mechanistic
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Assertion

In adolescent athletes, consuming caffeine before high-intensity endurance exercise raises heart rate if they have the ACE I allele (DI or II genotypes), but does not raise heart rate if they have the DD genotype.

Causal
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Assertion

Caffeine makes people feel like they are working harder during exercise and increases their motivation to work out, but it does not cause muscles to grow unless the physical load on the muscles is increased.

Causal
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