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The Study

Differential Time-of-Day Effects of Caffeine Capsule and Mouth Rinse on Cognitive Performance in Adolescent Male Volleyball Athletes: A Randomized Crossover Investigation

In simple terms

This study tested if caffeine helps teens think faster during volleyball practice, and it found that it does — but only for this specific group of boys who don’t usually drink caffeine. It doesn’t prove caffeine helps everyone, just these guys under these specific conditions.

64%

Analysis score

64/ 90

Maximum 90 for a randomized controlled trial.

Where the score came from

Reporting40
Methodology65
Publication100
Statistical54
Study type (basis of the score)
Randomized Controlled Trial
Level 1b - Individual RCT
What’s the bottom line?

This study tested if drinking caffeine or swishing it in your mouth helps teen volleyball players think and react faster at different times of day.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Randomized Trials
Level 1b
64

64 / 100

Quality score

Participants are randomly assigned to treatment or control groups, minimizing bias. The gold standard for testing whether an intervention causes an effect.

Can establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1These small speed improvements are like gaining a year of training — they could help athletes react faster during fast-paced volleyball rallies.
  2. 2Caffeine capsules made players react 9–11 milliseconds faster and cut thinking errors by 20–22% in the morning and midday.
  3. 3Mouth rinse helped only with thinking errors at midday (16–22% better), but not with reaction speed.
  4. 4No benefits were seen at night.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Life

Year

2025

Authors

Salma Belhaj Amor, Wissem Dhahbi, Houda Bougrine, Manel Bessifi, Vlad Adrian Geantă, Vasile Emil Ursu, Khaled Trabelsi, Nizar Souissi

Open Access
4 citations
Analysis v5

Related Content

Claims (5)

Assertion

In adolescent male volleyball players with low caffeine consumption, a 3 mg/kg caffeine capsule taken in the morning or midday improves reaction speed by 9–11 milliseconds and reduces cognitive interference by 20–22% during attention tasks.

Causal
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Assertion

Rinsing the mouth with a caffeine solution at 3 mg/kg improves performance on a cognitive task measuring mental interference during midday in adolescent male volleyball players, but does not improve reaction time on simpler tasks.

Mechanistic
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Assertion

Caffeine given as capsules or mouth rinses at 3 mg/kg does not consistently improve thinking performance in adolescent male volleyball players during evening training, even though their baseline performance is already high due to natural daily rhythms in alertness.

Mechanistic
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Assertion

Giving adolescent male volleyball players 3 mg of caffeine per kilogram of body weight does not change their oral temperature, and any cognitive effects from caffeine occur through direct brain mechanisms, not through changes in body heat.

Mechanistic
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Assertion

In adolescent male volleyball players, taking caffeine in capsule form leads to larger and more consistent improvements in reaction time and executive function during morning and midday sessions than using a caffeine mouth rinse, because absorption into the bloodstream produces stronger cognitive effects than stimulation in the mouth.

Causal
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