Blocking ER-α in liver cancer cells made them much less able to invade through membranes in lab tests, with about 75-80% fewer cells able to move through the barrier.
Scientific Claim
ER-α knockdown in Hep3B and HCCLM3 human hepatocellular carcinoma cell lines significantly reduced cell invasion capacity by approximately 75-80% compared to control groups (P < 0.05), with similar findings in both cell lines.
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study directly measured invasion capacity in cell cultures with quantitative metrics and statistical significance. The claim accurately reports the observed effects without overgeneralizing to human patients.
Source Excerpt
“In Hep3B cells, we found that ER-α siRNA infected cells showed a significant decreased invasion compared with cells in untreated group (50 ± 17 versus 201 ± 36, P = 0.021), LV group (50 ± 17, versus 214 ± 45, P = 0.016), and NS siRNA group (50 ± 17 versus 210 ± 34, P = 0.018) (Figure 4). NS siRNA group showed no significant differences in cell invasion compared with the control group (210 ± 34 versus 201 ± 36, P = 0.22) and LV group (210 ± 34 versus 214 ± 45, P = 0.034). Similar findings have been observed in HCCLM3 cells.”
Evidence from Studies
Supporting Evidence (1)
The study used transwell invasion assays to measure cell invasion capacity. The specific numerical values (e.g., 50 ± 17 vs 201 ± 36) and statistical significance (P < 0.05) demonstrate the reduction in invasion capability.
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