When using a control that doesn't block ER-α, there was no change in cancer cell growth, invasion, or death compared to normal cells.
Scientific Claim
ER-α knockdown in Hep3B and HCCLM3 human hepatocellular carcinoma cell lines did not significantly affect cell proliferation, invasion, or apoptosis when using nonsilencing siRNA or lentivirus alone as controls.
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study included appropriate control groups and demonstrated no significant effects in these controls. The claim accurately reports the lack of effect in control conditions.
Source Excerpt
“whereas no significant inhibitory effect was observed in cells treated with NS siRNA, LV, or untreated cells (Figure 2(a)). NS siRNA group showed no significant differences in cell invasion compared with the control group (210 ± 34 versus 201 ± 36, P = 0.22) and LV group (210 ± 34 versus 214 ± 45, P = 0.034). Compared with the control group and LV group, NS siRNA did not significantly increase cell apoptosis (P > 0.05, resp.).”
Evidence from Studies
Supporting Evidence (1)
The study included proper control groups (NS siRNA, LV alone) and demonstrated no significant effects on proliferation, invasion, or apoptosis compared to untreated cells, confirming that the observed effects were specifically due to ER-α knockdown.
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