In mice with high blood pressure, the sodium channel protein in the kidneys was more than double in both kidneys, but a drug called PRO20 reduced this increase to about 1.5 times and 1.2 times in the clipped and other kidney.
Scientific Claim
In 2K1C Goldblatt mice, αENaC protein expression increased 2.2-fold in clipped kidneys and 2.6-fold in non-clipped kidneys compared to sham controls; PRO20 treatment was associated with lower αENaC expression (1.5-fold and 1.2-fold increases, respectively).
Original Statement
“The left kidneys of 2K1C mice showed increased abundance of αENaC (fold change in control [sham]: 2.2 ± 0.2 vs. 1.0 ± 0.1, p < 0.01, Figure 5A) as well as in right kidneys (fold change in control: 2.6 ± 0.1 vs. 1.0 ± 0.1, p < 0.01, Figure 5B). Interestingly, the increases in protein abundance of αENaC were only partially prevented in the left kidneys of mice subjected to 2K1C surgery and systemically infused with PRO20 for 7 days (fold change in control [sham]: 1.5 ± 0.1 vs. 1.0 ± 0.1, p < 0.05, Figure 5A); however, in the right kidneys PRO20 treatment blunted the increased abundance of αENaC observed in kidneys from 2K1C mice (fold change in control [sham]: 1.2 ± 0.1 vs. 1.0 ± 0.1, p < 0.05, Figure 5B).”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
association
Can only show association/correlation
Assessment Explanation
The claim correctly uses 'increased' for the descriptive measurement and 'associated with' for the PRO20 effect, which aligns with the study's observational design.
Evidence from Studies
Supporting (1)
(Pro)renin Receptor Blockade Prevents Increases in Systolic Blood Pressure, Sodium Retention, and αENaC Protein Expression in the Kidney of 2K1C Goldblatt Mice