mechanistic
Analysis v1
14
Pro
0
Against

In rat heart arteries, the ketone body relaxes blood vessels without needing the inner lining or certain inflammatory chemicals.

Scientific Claim

In isolated rat coronary arteries, sodium 3-hydroxybutyrate-induced vasorelaxation was endothelium-independent and not mediated by cyclooxygenase-dependent prostanoids.

Original Statement

After denudation of the endothelial cell layer—achieved by passing air bubbles through the lumen—the coronary arteries, as expected, did not relax in response to 5 µM acetylcholine (Fig. 3E). However, the endothelial denudation did not restrict the relaxation to 10 mM 3-OHB (Fig. 3F). We also observed no influence on the 3-OHB-induced vasorelaxation when coronary arteries were pre-incubated with 3 µM indomethacin (Fig. 3G), supporting that a cyclooxygenase-dependent prostanoid is not involved.

Evidence Quality Assessment

Claim Status

appropriately stated

Study Design Support

Design supports claim

Appropriate Language Strength

definitive

Can make definitive causal claims

Assessment Explanation

The targeted mechanistic experiments in isolated tissues support definitive statements about the underlying process.

Evidence from Studies

Contradicting (0)

0
No contradicting evidence found