View

The Study

Moderate exercise induces trained immunity in macrophages.

In simple terms

This study looked at how exercise changed the behavior of immune cells in mice, but only in a lab dish—not in living animals or people. It shows a connection between exercise and cell changes, but doesn't prove exercise causes those changes to happen in real life or in humans.

18%

Analysis score

18/ 72

Maximum 72 for a cohort study.

Where the score came from

Reporting40
Methodology57
Publication100
Statistical54
Study type (basis of the score)
Cohort Study
Level 2b - Individual cohort study
What’s the bottom line?

When mice exercise regularly, their immune cells in the bone marrow learn to be less angry when they sense germs — they stop overreacting and start using oxygen better instead of burning sugar.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Cohort Studies
Level 2b
18

18 / 100

Quality score

Groups of people are followed over time to see who develops an outcome. Strong for identifying risk factors and associations, but cannot prove causation as firmly as RCTs.

Cannot establish causation

Save studies & get personalized insights

Create a free account to save this study, track new evidence as it comes in, and get breakdowns of studies in the topics you care about.

Key takeaways

Summary

Based on the study abstract and findings.

  1. 1This means regular exercise might help prevent chronic diseases caused by too much inflammation, like arthritis or diabetes, by training immune cells to stay calm longer.
  2. 2Exercised mice had 30–50% less inflammatory signals (like TNF-α) and 2–3x more mitochondrial efficiency in immune cells.
  3. 3Their DNA opened up near anti-inflammatory genes and closed near inflammatory ones.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

American journal of physiology. Cell physiology

Year

2023

Authors

Mayoorey Murugathasan, Ardavan Jafari, A. Amandeep, S. A. Hassan, Matthew Chihata, A. Abdul-Sater

38 citations
Analysis v6

Related Content

Claims (6)

Assertion

Moderate-intensity aerobic exercise lowers the activity of inflammatory genes in immune cells by altering epigenetic marks or gene transcription mechanisms.

Mechanistic
Read analysis
Assertion

In mice, regular moderate exercise causes lasting changes in immune cells from the bone marrow that reduce inflammatory responses to bacterial signals and enhance energy production pathways.

Mechanistic
Read analysis
Assertion

In mice, moderate exercise improves mitochondrial function in bone marrow-derived macrophages by lowering reactive oxygen species production, increasing membrane potential, and increasing the number of functional mitochondria, which shifts cellular metabolism toward oxidative phosphorylation.

Mechanistic
Read analysis
Assertion

In mice, regular moderate exercise changes the accessibility of DNA regions that control anti-inflammatory and pro-inflammatory genes, leading to a shift in macrophage gene expression patterns toward an anti-inflammatory state.

Mechanistic
Read analysis
Assertion

In mice, moderate exercise decreases the use of glycolysis in macrophages activated for inflammation and increases the use of oxidative phosphorylation in macrophages activated for tissue repair, leading to a metabolic profile associated with reduced inflammation.

Mechanistic
Read analysis
Assertion

In mice, moderate exercise changes the metabolism and inflammation profile of immune cells from the bone marrow; after two weeks without exercise, most changes reverse, but reduced glycolysis remains.

Mechanistic
Read analysis
Fit Body Science verdict — we translate health studies into clear verdicts backed by peer-reviewed research.

Not medical advice. For informational purposes only. Always consult a qualified healthcare professional before making health decisions.