A tiny amount of methylene blue helps heart cells make energy better, but too much actually hurts them — it’s a classic case of 'less is more'.
Scientific Claim
In isolated rat heart mitochondria, the beneficial effect of methylene blue on respiration is observed only at a low concentration (0.1 µM), with higher concentrations (1–10 µM) shown in prior studies to suppress respiration, suggesting a hormetic dose-response.
Original Statement
“In line with the previously mentioned hormetic behavior, it is important to emphasize that the beneficial effects of MB were observed at very low doses (i.e., 0.1 µM). In high concentrations, it has been speculated that MB could 'steal' electrons from the ETC complexes resulting in a disrupted redox balance.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study explicitly references prior data showing dose-dependent reversal and tests only the effective low dose; the hormetic claim is supported by external evidence cited within the study’s own framework.
Evidence from Studies
Supporting (1)
At a very low dose, methylene blue helps heart mitochondria work better, which matches the claim — higher doses might hurt, but this study only tested the good low dose and found it works.