Adding methylene blue doesn’t change how well heart cell power plants can hold onto calcium, meaning it doesn’t protect against the pore that can cause cell death under stress.
Scientific Claim
In isolated rat heart mitochondria, 0.1 µM methylene blue does not alter calcium retention capacity, indicating no direct effect on mitochondrial permeability transition pore sensitivity in either diabetic or non-diabetic conditions.
Original Statement
“No changes in the calcium retention capacity of healthy or diabetic mitochondria were found in the presence of methylene blue.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study directly measured calcium retention capacity under controlled conditions and found no effect; the absence of change is clearly reported and statistically validated.
Evidence from Studies
Supporting (1)
The study found that a tiny amount of methylene blue didn’t change how well mitochondria held onto calcium, which means it didn’t make the pore that can cause cell damage more or less likely to open — whether the rats were diabetic or not.