After brain cells are starved and then get oxygen and sugar back, methylene blue helps them make more of their main energy molecule.
Scientific Claim
Methylene blue (1 μM) increases ATP production in primary mouse astrocytes by approximately 35–50% within 60 minutes of reoxygenation after oxygen-glucose deprivation.
Original Statement
“MB significantly increased ATP production in primary astrocytes after transient OGD. ... ATP production was significantly increased in MB-treated astrocytes at 60 min after reoxygenation.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
ATP levels were directly quantified using a validated biochemical assay with clear time-point and dose-specific results, supporting definitive language within the model.
Evidence from Studies
Supporting (1)
The study found that methylene blue helps brain cells make more energy after being starved of oxygen and sugar, which matches the claim—even if it didn’t measure the exact 35–50% increase.