After damaging the eye tissue with enzymes, adding a saline solution (vehicle) made the stiffness drop even more by about 26% compared to the damaged state.

When rats' eye tissue was treated with an enzyme that breaks down collagen, the stiffness of the outer part of the eye decreased by almost 40% compared to normal.

After damaging the eye tissue with collagen-breaking enzymes, applying a special peptide helped restore some stiffness, increasing it by about 23% compared to the damaged state.

After damaging the inner optic nerve head tissue with enzymes, adding saline solution slightly increased stiffness by about 13% compared to the damaged state.