Animal studies investigating artificial sweeteners frequently employ pharmacologically extreme dosages, which are then extrapolated to humans using a 100-fold safety margin, a practice that does not align with realistic human exposure levels.

Study: Consumption of Artificial Sweetener Acesulfame Potassium Increases Preterm Risk and Uterine Contraction with Calcium Influx increased via Myosin Light Chain kinase - myosin Light Chain 20 related Signaling Pathway.

This study gave animals a lot more artificial sweetener than people usually eat, then used that to guess what might happen to humans — which is exactly what the claim says happens.

Study: Impact of Artificial Sweeteners on Inflammation Markers: A Systematic Review of Animal Studies

This study looked at how animals reacted to artificial sweeteners at high doses — much higher than what people usually eat — and found possible health effects. This matches the claim that animal studies use crazy-high doses that don’t reflect real human habits.

Study: Intestinal Metabolism and Bioaccumulation of Sucralose In Adipose Tissue In The Rat

Scientists gave rats a lot of sucralose—way more than humans ever eat—and found the sweetener stuck in their fat and changed in ways no one knew about. This supports the idea that animal studies use crazy-high doses that don’t match how much people actually consume.

Study: Artificial sweeteners and brain health: critical evaluation of aspartame impact on neurovascular and cognitive consequences

This study says that even the amount of artificial sweetener deemed 'safe' for humans might still harm the brain, especially in kids or people with health problems — which means the safety rules might be too loose, just like the claim says.