In mice with insulin resistance, giving extra insulin for two weeks lowered the levels of nitric oxide breakdown products in blood and urine by almost half, even though the kidney showed signs of producing more nitric oxide.

Scientific Claim

Chronic insulin infusion in TALLYHO/Jng mice was associated with a 40-50% reduction in plasma and urinary nitrate/nitrite (NOx) levels after 14 days of treatment, despite increased endothelial nitric oxide synthase phosphorylation and activity in the kidney.

Original Statement

“plasma and urine levels of nitrates plus nitrites (NOx) fell with insulin infusion, by day 14 (40–50%)”

From study:Chronic Insulin Infusion Down-Regulates Circulating and Urinary Nitric Oxide (NO) Levels Despite Molecular Changes in the Kidney Predicting Greater Endothelial NO Synthase Activity in Mice

Evidence Quality Assessment

Claim Status

appropriately stated

Study Design Support

Design supports claim

Appropriate Language Strength

association

Can only show association/correlation

Assessment Explanation

The study is an animal cohort study that measures associations between insulin infusion and NOx levels. The verb 'was associated with' correctly reflects the observational nature of the data without implying causation.

Evidence from Studies

Supporting (1)

Chronic Insulin Infusion Down-Regulates Circulating and Urinary Nitric Oxide (NO) Levels Despite Molecular Changes in the Kidney Predicting Greater Endothelial NO Synthase Activity in Mice

Cohort Study

Animal

2018 Sep 22

Contradicting (0)

No contradicting evidence found

Source Study

Chronic Insulin Infusion Down-Regulates Circulating and Urinary Nitric Oxide (NO) Levels Despite Molecular Changes in the Kidney Predicting Greater Endothelial NO Synthase Activity in Mice

Score: 13

DOI: 10.3390/ijms19102880

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