Methylene blue fixes a key enzyme that helps brain cells start using sugar for energy after they’ve been starved.
Scientific Claim
Methylene blue (1 μM) restores hexokinase activity in primary mouse astrocytes following oxygen-glucose deprivation, reversing the 30–40% reduction caused by ischemic insult.
Original Statement
“MB treatment prevents the reduction and maintains the hexokinase activity which improves glucose metabolism following OGD-reoxygenation. ... MB significantly restored OGD induced loss of hexokinase activity.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The study directly measured enzyme activity in cell lysates with appropriate controls and statistical analysis, supporting a definitive claim within the model.
Evidence from Studies
Supporting (0)
Contradicting (1)
The study shows methylene blue helps brain cells survive after being starved of oxygen and sugar, but it never checked if the specific enzyme (hexokinase) mentioned in the claim got better, so we can't say for sure if that part is true.