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The Study

Effects of high versus standard essential amino acid intakes on whole-body protein turnover and mixed muscle protein synthesis during energy deficit: A randomized, crossover study.

In simple terms

This study gave two different amounts of amino acid drinks to 19 young men after they ate less food than usual, then measured how their bodies used protein. It shows that the bigger dose made their whole body use protein better, but didn’t make their muscles grow more. It’s like testing two types of fertilizer on the same plants — we know which one helped the plant overall, but not which helped the leaves more.

72%

Analysis score

72/ 90

Maximum 90 for a randomized controlled trial.

Where the score came from

Reporting0
Methodology77
Publication100
Statistical100
Study type (basis of the score)
Randomized Controlled Trial
Level 1b - Individual RCT
What’s the bottom line?

When people eat less food and exercise, giving them more amino acids helps their whole body make protein and stop breaking it down—but doesn’t make their muscles grow faster.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Randomized Trials
Level 1b
72

72 / 100

Quality score

Participants are randomly assigned to treatment or control groups, minimizing bias. The gold standard for testing whether an intervention causes an effect.

Can establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1Yes—this means more amino acids help your body stay in repair mode during dieting, even if your muscles don’t grow faster from the extra dose.
  2. 2With 23.5g EAA: protein synthesis went up by 3.4g/180min, breakdown went down by 15.6g/180min, net balance improved by 19.0g/180min.
  3. 3Muscle protein synthesis rates were the same with 7.87g or 23.5g EAA.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Clinical nutrition

Year

2020

Authors

Jess A Gwin, D. Church, Adrienne Hatch-McChesney, Emily E. Howard, C. Carrigan, Nancy E. Murphy, Marques A. Wilson, Lee M. Margolis, J. Carbone, R. Wolfe, A. Ferrando, S. Pasiakos

Open Access
25 citations
Analysis v5

Related Content

Claims (7)

Assertion

In young men who are in a calorie deficit, taking a higher dose of essential amino acids after weight training does not lead to a higher rate of muscle protein synthesis compared to a lower dose.

Quantitative
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Assertion

When young adult men are in a calorie-deficient state, consuming 23.5 grams of essential amino acids leads to higher levels of these amino acids in the blood than consuming 7.87 grams.

Quantitative
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Assertion

When the body consumes significantly fewer calories than it expends, muscle protein synthesis decreases and muscle protein breakdown increases, resulting in reduced muscle growth.

Mechanistic
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Assertion

In young adult males with a 30% energy deficit over five days, consuming 23.5 grams of essential amino acids after resistance exercise increases whole-body protein synthesis by 3.4 grams per 180 minutes and decreases protein breakdown by 15.6 grams per 180 minutes compared to 7.87 grams, leading to a more positive net protein balance, with no change in muscle protein synthesis rates.

Mechanistic
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Assertion

In young men on a 5-day low-calorie diet, taking a higher dose of essential amino acids (23.5 grams) compared to a lower dose (7.87 grams) increases overall protein synthesis and decreases protein breakdown, leading to a better net protein balance, but does not increase muscle protein synthesis during rest or after weight training.

Causal
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Assertion

In young men eating fewer calories than they burn, taking either a low or high dose of essential amino acids results in the same rate of muscle protein synthesis during rest and after weight training.

Causal
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