Apelin activates a key energy-sensing enzyme in muscles, which helps reduce fat buildup and improve metabolism.
Scientific Claim
Chronic apelin treatment (0.1 μmol/kg/day intraperitoneally for 4 weeks) in high-fat diet-induced insulin-resistant mice is associated with increased AMPK phosphorylation and reduced malonyl-CoA concentrations in skeletal muscle (n=3-4 per group, P < 0.05).
Original Statement
“Apelin treatment significantly increased both AMPK and ACC phosphorylation in muscle of insulin-resistant mice (Fig. 5A). ... the inhibition of ACC activity (as a result of increased phosphorylation) had for consequence a significant reduction of malonyl-CoA concentrations in muscle of apelin-treated mice (Fig. 5B).”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
association
Can only show association/correlation
Assessment Explanation
The claim uses 'associated with' which correctly reflects the associative nature of the study design, avoiding causal language.
Evidence from Studies
Supporting (1)
Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice