MOTS-c treatment reduced collagen buildup in the muscles of immobilized mice, which may help prevent stiffness.

Scientific Claim

In male C57BL/6J mice with 8 days of hindlimb immobilization, daily MOTS-c administration (15 mg/kg/day) was associated with lower skeletal muscle collagen levels compared to untreated controls.

Original Statement

“Skeletal muscle total collagen levels were analyzed in the gastrocnemius muscle with the Total Collagen Assay Kit (Abcam), following the manufacturer’s protocol.”

From study:Mitochondrial-derived microprotein MOTS-c attenuates immobilization-induced skeletal muscle atrophy by suppressing lipid infiltration.

Evidence Quality Assessment

Claim Status

understated

Study Design Support

Design supports claim

Appropriate Language Strength

association

Can only show association/correlation

Assessment Explanation

The study did not report collagen results in the main text, so the claim should reflect the absence of data rather than a specific finding.

More Accurate Statement

“The study measured skeletal muscle collagen levels in male C57BL/6J mice with 8 days of hindlimb immobilization but did not report significant differences between MOTS-c-treated and control groups.”

Evidence from Studies

Supporting (1)

Mitochondrial-derived microprotein MOTS-c attenuates immobilization-induced skeletal muscle atrophy by suppressing lipid infiltration.

Randomized Controlled Trial

Animal

2024 Mar 1

Contradicting (0)

No contradicting evidence found

Source Study

Mitochondrial-derived microprotein MOTS-c attenuates immobilization-induced skeletal muscle atrophy by suppressing lipid infiltration.

Score: 16

DOI: 10.1152/ajpendo.00285.2023

Similar Assertions

When mice had their legs immobilized for 8 days, giving them MOTS-c helped their muscles lose less weight (5% instead of 15%) compared to mice that didn't get the treatment.

89%

MOTS-c lowered levels of myostatin, a protein that inhibits muscle growth, in the muscles of immobilized mice.

88%

MOTS-c reduced the amount of fatty acids in the muscles of immobilized mice, which may prevent harmful fat buildup.

86%

In immobilized mice, higher levels of MOTS-c in the muscles were linked to better muscle mass retention.

86%

When mice had their legs immobilized for 8 days, giving them MOTS-c daily helped their muscles lose less weight—only 5% compared to 15% without treatment.

85%