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The Study

Polyphenol-mediated microbiome modulation in STEMI patients: a pilot study

In simple terms

This study gave some heart attack patients a grape extract and others a fake one, then checked their gut bacteria. It found that the grape extract group had different bacteria and didn't have rising TMAO levels. But it doesn't prove the grape extract caused these changes—it just shows they happened together.

81%

Analysis score

81/ 90

Maximum 90 for a randomized controlled trial.

Where the score came from

Reporting40
Methodology80
Publication100
Statistical100
Study type (basis of the score)
Randomized Controlled Trial
Level 1b - Individual RCT
What’s the bottom line?

After a heart attack, some gut bacteria make a chemical called TMAO that can harm your heart. This study gave people a grape juice concentrate to see if it could change their gut bacteria to be healthier.

Where does this study sit?

Reviews of RCTs (Meta-analyses)

Max 100

Randomized Trials

Max 90

Reviews of Cohort Studies

Max 85

Cohort Studies

Max 72

Reviews of Case-Control Studies

Max 63

Case-Control Studies

Max 58

Cross-Sectional & Case Series

Max 50

Expert Opinion

Max 5
StrongerWeaker
Randomized Trials
Level 1b
81

81 / 100

Quality score

Participants are randomly assigned to treatment or control groups, minimizing bias. The gold standard for testing whether an intervention causes an effect.

Can establish causation

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Key takeaways

Summary

Based on the study abstract and findings.

  1. 1This suggests the grape juice may help protect the heart by changing gut bacteria to make less harmful TMAO — but it’s not proven to prevent future heart problems yet.
  2. 2People who drank the grape concentrate had stable TMAO levels (no increase), while those who drank placebo had TMAO levels double.
  3. 3Their good gut bacteria (Roseburia, Agathobaculum, Alistipes finegoldii, Sellimonas) went up, and bad ones (Alistipes timonensis, Parabacteroides goldsteinii) went down.

Score breakdown, methodology, conflicts of interest, evidence analysis & raw study data

Publication

Journal

Frontiers in Medicine

Year

2025

Authors

A. Issilbayeva, S. Sergazy, A. Zhashkeyev, A. Gulyayev, S. Kozhakhmetov, Z. Shulgau, M. Nurgaziyev, A. Nurgaziyeva, Sanzhar Zhetkenev, N. Mukhanbetzhanov, Zharkyn Jarmukhanov, Zhanel Mukhanbetzhanova, E. Vinogradova, Z. Zhumadilov, A. Kushugulova, M. Aljofan

Open Access
2 citations
Analysis v5

Related Content

Claims (6)

Assertion

Consuming colorful plant foods provides polyphenols that directly increase the activity and diversity of gut microbes.

Mechanistic
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Assertion

In patients with STEMI, polyphenol supplementation is linked to higher levels of Alistipes finegoldii and lower levels of Alistipes timonensis; higher Alistipes finegoldii levels are associated with lower plasma TMAO levels, while higher Alistipes timonensis levels are associated with higher plasma TMAO levels.

Correlational
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Assertion

In people who have had a heart attack called STEMI, taking polyphenol supplements is linked to higher levels of two gut bacteria, Sellimonas and Agathobaculum, that produce butyrate and are associated with reduced activity of bacteria that make TMAO.

Correlational
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Assertion

In patients who have had a specific type of heart attack, taking 15 mL of grape polyphenol concentrate daily for three months is linked to no change in plasma TMAO levels, while those taking a placebo show a measurable increase in plasma TMAO levels.

Correlational
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Assertion

In patients who have had a heart attack called STEMI, taking polyphenol supplements for three months is linked to a decrease in the ratio of Firmicutes to Bacteroidetes gut bacteria and an increase in specific butyrate-producing bacteria.

Correlational
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Assertion

In patients who have had a heart attack called STEMI, taking polyphenol supplements is linked to lower levels of two gut bacteria, Parabacteroides goldsteinii and Lactobacillus salivarius, which have been connected to the production of trimethylamine N-oxide and lipid metabolism.

Correlational
Read analysis
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