Apelin-treated mice burn more fat for energy during active periods compared to placebo-treated mice, shown by their breathing patterns.
Scientific Claim
Chronic apelin treatment (0.1 μmol/kg/day intraperitoneally for 4 weeks) in high-fat diet-induced insulin-resistant mice is associated with enhanced lipid oxidation in vivo, as indicated by lower respiratory exchange ratio during the dark cycle (feeding period) (n=12 apelin vs n=13 PBS, P ≤ 0.05).
Original Statement
“apelin-treated mice clearly exhibited a lower RER, especially during the feeding period (dark cycle) compared with PBS-treated mice (Fig. 2A and B), indicative of a higher use of lipids. These results were confirmed by the calculated amount of lipid oxidized, which was increased, whereas the amount of glucose oxidized was decreased in HFD apelin-treated mice (Fig. 2C).”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
association
Can only show association/correlation
Assessment Explanation
The claim uses 'associated with' which correctly reflects the associative nature of the study design, avoiding causal language.
Evidence from Studies
Supporting (1)
Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice