Mice given MOTS-c had more ANGPTL4 protein in their muscles and less fatty acids, which may help prevent muscle damage from fat buildup.
Scientific Claim
In male C57BL/6J mice with 8 days of hindlimb immobilization, daily MOTS-c administration (15 mg/kg/day) was associated with higher skeletal muscle ANGPTL4 protein expression and lower fatty acid levels compared to untreated controls.
Original Statement
“The protein expression levels of ANGPTL4... were higher in the MOTS-c-treated group than in the immobilization control group (Fig. 5B). Furthermore, skeletal muscle fatty acid levels were significantly lower in the MOTS-c-treated group than in the immobilization control group (P < 0.05).”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
association
Can only show association/correlation
Assessment Explanation
The claim uses 'was associated with' and specifies the model and measurements without causal language, appropriate for the study design.
Evidence from Studies
Supporting (1)
Mitochondrial-derived microprotein MOTS-c attenuates immobilization-induced skeletal muscle atrophy by suppressing lipid infiltration.