MOTS-c increased the production of a protein called ANGPTL4 in the muscles of immobilized mice, which helps control fat uptake into muscle tissue.

Mice given MOTS-c had more ANGPTL4 protein in their muscles and less fatty acids, which may help prevent muscle damage from fat buildup.

MOTS-c lowered levels of myostatin, a protein that inhibits muscle growth, in the muscles of immobilized mice.

When mice had their legs immobilized for 8 days, giving them MOTS-c helped their muscles lose less weight (5% instead of 15%) compared to mice that didn't get the treatment.

In immobilized mice, higher levels of MOTS-c in the muscles were linked to better muscle mass retention.

MOTS-c reduced the amount of fatty acids in the muscles of immobilized mice, which may prevent harmful fat buildup.