Obese mice treated with MOTS-c had lower levels of ANGPTL4 in their muscles, which may help prevent fat buildup.

MOTS-c treatment in obese mice reduced the activity of a liver enzyme called Por by half, which may affect fat metabolism.

Mice given MOTS-c had more ANGPTL4 protein in their muscles and less fatty acids, which may help prevent muscle damage from fat buildup.

MOTS-c increased the production of a protein called ANGPTL4 in the muscles of immobilized mice, which helps control fat uptake into muscle tissue.

Obese mice given MOTS-c had lower levels of suberate, a dicarboxylic acid, by 40% compared to untreated mice.

MOTS-c injections made muscle cells more responsive to insulin by activating AMPK and increasing GLUT4, which helps move glucose into cells.