When the fat tissue was given serotonin (a chemical that MAO-A breaks down), it made more harmful molecules—but adding methylene blue stopped some of that increase.
Scientific Claim
Exposure of human epicardial and perivascular adipose tissue to serotonin (10 µM) increases hydrogen peroxide production, and this increase is partially reversed by co-incubation with 0.1 µM methylene blue, suggesting MAO-A contributes to substrate-driven oxidative stress in these tissues.
Original Statement
“Incubation with SR more than doubled the H2O2 production in both types of adipose tissue, an effect that was partially reversed by MB.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
definitive
Can make definitive causal claims
Assessment Explanation
The claim describes a direct experimental manipulation and observed outcome in ex vivo tissue. The language reflects the measured effect without overextending to in vivo or clinical relevance.
Evidence from Studies
Supporting (1)
Methylene blue reduces monoamine oxidase expression and oxidative stress in human cardiovascular adipose tissue
The study found that serotonin makes fat around the heart produce more harmful chemicals, and a drug called methylene blue can partly stop this — suggesting serotonin works through an enzyme called MAO-A to cause the damage.