MOTS-c changed the activity of genes related to fat metabolism and PPAR pathways in the muscles of immobilized mice, which may affect how fat builds up in muscle tissue.
Scientific Claim
In male C57BL/6J mice with 8 days of hindlimb cast immobilization, MOTS-c administration (15 mg/kg/day) was associated with altered expression of genes involved in adipogenesis and peroxisome proliferator-activated receptor (PPAR) signaling pathways in skeletal muscle.
Original Statement
“Unbiased RNA sequencing and its downstream analyses demonstrated that MOTS-c modified adipogenesis-modulating gene expression within the peroxisome proliferator-activated receptor (PPAR) pathway.”
Evidence Quality Assessment
Claim Status
appropriately stated
Study Design Support
Design supports claim
Appropriate Language Strength
association
Can only show association/correlation
Assessment Explanation
The study is an animal cohort with no blinding confirmation, so causal language is inappropriate. The claim uses 'associated with' which correctly reflects the associative evidence.
Evidence from Studies
Supporting (1)
Mitochondrial-derived microprotein MOTS-c attenuates immobilization-induced skeletal muscle atrophy by suppressing lipid infiltration.